Gene Expression

Gene Expression

Introduction

ORB provides Gene Expression Analysis utilizing microarrays from the major manufacturer's including Agilent, Affymetrix, and Microarrays, Inc. ORB can also handle hybridizations for your ChIP on Chip, Methylation, and Comparative Genomic Hybridization (CGH) studies. In addition, ORB also provides follow-up validation of microarray results using the ABI Taqman platform, as well as comprehensive data and pathway analysis services.

Our mission is to provide you with reproducible, sensitive, and accurate microarray results at a competitive price. In addition, we are committed to excellent customer service, full documentation of every phase of the project, and prompt service. Turn-around is 15 days or less for most projects. As part of our standard service we provide initial consultation on experimental design, and quality control reporting at multiple project phases. We offer modular analysis packages that ensure you pay for only the data analysis you require. At the same time we provide custom analysis (e.g. mRNA-microRNA expression correlation analysis) at very affordable prices. Follow-up support includes preparation of images for posters and grants, customized text for your methods section, and training on the use of pathway analysis software.

Description of Services

ORB provides complete processing services for microarrays produced by multiple vendors according to the manufacturer's recommended protocols. Choose from any of the available microarrays listed in Table I. Detailed descriptions are available by clicking on the array type.

The Standard Processing Service Includes:

  • Spectrophotometric & electrophoretic analysis of RNA samples, with the option to replace samples at no additional cost.
  • Amplification and labeling of cRNA and cDNA and QC testing of the amplified product.
  • Hybridization, feature extraction, and basic data processing.
  • A quality control report including multiple measures of signal-to-noise and reproducibility for each microarray hybridized.

Array processing is just $325 per sample for one-color hybridization*. To determine entire project price just add the cost of your selected microarrays and data analysis modules. See our 2014 Price List for complete pricing information. Don't forget to Contact Us to find out about current specials and discounts!

*Minimum 12 samples, see price sheet for other quantities.

Data Analysis Modules

  • Standard Data Analysis: Normalization, fold-change calculations, analysis of variance (ANOVA), hierarchical clustering, principal component analysis (3 or more treatment groups), and power point summary.
  • Basic Pathway Analysis: Examine over-representation of differentially expressed genes based on up to 4 fold-change criteria in KEGG and Biocarta functional pathways. Excel Tables.
  • Extended Pathway Analysis: Examine over-representation of differentially expressed genes based on up to 8 fold-change criteria in KEGG, Biocarta, and GO functional groups using two different software applications. Excel and power point summaries included.
  • Custom Data Analysis: MicroRNA-mRNA anti-correlation analysis, Exon-level analysis, and more (please inquire).

Table I. Available Microarray Platforms

Array Type Array Type Highlights
Exonic Evidence-Based Oligonucleotide Microarrays
Human - HEEBO
Mouse - MEEBO
State of the art
70-mer oligonucleotide deposition
  • Human 48.5K or Mouse 38.5K Whole Genome
  • Full coverage of Refseq transcripts with additional coverage of splice variants
  • Extensive set of quality control features for robust data analysis
  • See Exonic Evidence Page for more information

AffymetrixSee Affymetrix PageSee Affymetrix Page

Agilent Whole Genome Microarrays (External Link)SurePrint in situ oligo synthesis technology
  • Human, Mouse, or Rat 44K feature arrays
  • Other Model Organisms
  • Validated by FDA Quality Control Consortium More Information
Agilent CGH & ChIP on Chip Microarrays (External Link)SurePrint in situ oligo synthesis technology
  • Human (244K, 105K, and 44K), Mouse (244K, 105K, and 44K), Rat (244K and 105K), and Chicken (244K) CGH microarrays
  • Human and Mouse 244K Promoter as well as 244K Whole Genome tiling arrays (multiple species) for ChIP on Chip
More Information

Custom Arrays
(Please Ask Us)
State of the art oligonucleotide deposition microarray technology1000 to 3000 features

For complete pricing information, please view the 2014 Price List. Contact us to inquire about current specials and discounts.

We also offer:

Contact Us

Ocean Ridge Biosciences
10475 Riverside Drive, Suite 1
Palm Beach Gardens, FL 33410
Phone: 561-223-3152
Fax: 561-427-7845
Email: array@oceanridgebio.com