ORB provides open-platform gene expression profiling on the Illumina HiSeq2000 Sequencing System with coverage of over 50 million 51 nucleotide sequence reads per sample in the standard service. Alternatively, ORB can increase the level of multiplexing of RNASeq libraries on the instrument in order to provide a low cost solution. ORB uses Illumina's TruSeq chemistry for generating unbiased cDNA libraries with high coverage uniformity and requires only 200 nanograms of total RNA. ORB backs up our laboratory mRNA sequencing service with affordable standard and custom data analysis packages.
Illumina RNA-Seq Process
Highlights
- Standard delivery of over 50 million 51 nt reads per sample
- Unbiased Illumina's Truseq chemistry based libraries
- Competitively priced library preparation, sequencing, and data analysis services
- Multiplexing for cost savings
- Fully customizable sequencing depth and read length
- Complete bioinformatics support including statistical analysis
ORB will support your special sequencing needs including targeted resequencing, chip sequencing, exome sequencing and is willing to partner with you on custom projects. Please contact us with a brief description of your custom project needs.
Comprehensive Service Offerings Include:
- RNA sample quality control and report
- Library preparation
- Data QC and Report
- FASTQ sequence files and BAM alignment files for each sample
- Standard data analysis packages include: counts of mapped sequence reads (genes, exons, and splice junctions), tables of called SNPs, table of insertions/deletions, comprehensive statistical analysis, pathway analysis, and links to USC browser session for viewing tracks of sequence reads on the genome scaffold.
- Advanced data analysis and presentation capabilities including alternative splicing analysis, linear and logistic regression modeling, and generation of predictive models.
Pricing Information
| Minimum Reads | ++Single Read | ++Paired-End |
|---|---|---|
| 50 million | $2,144 | $2,914 |
| 25 million | $1,072 | $1,457 |
| 12.5 million | $536 | $728 |
++Displayed pricing includes library QC, data QC and report, sequencing at indicated depth, raw data processing, and delivery of FASTQ sequence and BAM alignment files. Library preparation and data analysis packages are additional.
To request a quote for your RNA-Seq project, please call 561-427-7845 or utilize the ORB Contact Form.