|
Ocean Ridge Biosciences
Multiplex Protein Profiling Assays on Luminex
|
|
 |
|
 |
|
 |
 |
Multiplex Protein Profiling Assays on Luminex | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|
Luminex xMAP technology enables the profiling of up to 100 different analytes in a single multiplex reaction. Luminex Corp. has developed a standard set of 100 polystyrene microspheres, each imbedded with specific ratios of two different fluorecent dyes. The ratio of the two dyes gives each bead type a unique specific identification of that bead in a mixture of other beads. (Fig. 1)
It is possible to design multiplexed protein profiling assays for the xMAP technology platform. Several hundred assay kits are already available. In the typical xMAP cytokine assay, specific bead types are each assigned to a different cytokine and coupled to primary antibodies directed against unique cytokine molecules. The bead mixtures are incubated with serum or cell culture supernatants in filter plates. The beads are then washed and incubated successively with biotnylated antibodies against each cytokine and with streptavadin-phycoerythrin. Mixtures of beads are injected in to the Luminex LX100 instrument, a modified flow-cytometer, wherein the type and levels of each analyte can be determined by laser-induced fluorescence. (Fig. 2) |
Figure 1: Impregnation of polystyrene beads with specific concentrations of two different red-emitting fluorophores allows the specific identification of 100 different bead types.
Figure 2: Dual lasers in the LX100 instrument excite the identification flurophores within each bead as well as the phycoerythrin fluorescent detection protein on the bead surface.
|
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|
ORB Protein Profiling Service
We offer accurate quantification of cytokines, chemokines, phosphorylated proteins, MMPs, growth factors, and receptors in Human, Mouse, Rat, and Monkey using Multiplex Bead-Based Luminex® kits from Invitrogen and R&D systems. With a rapid turn-around time of five business days, we strive to accelerate your research with reliable data using duplicate determination. We offer quantitation services on multiple sample types including serum, plasma, cell lysates, tissue culture supernants and even cerebrospinal fluid. Our service includes a standard analysis with Starstation software (free of charge). We use only kits that are calibrated to NIBSC standards, an international reference standard, when available.
|
Service Highlights
|
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Human Cytokine Twenty-Five-Plex [Pricing] | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|
We offer the simultaneous quantitation of human IL-1β, IL-1RA, IL-2, IL-2R, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12 (p40), IL-13, IL-15, IL-17, TNF-α, IFN-α, IFN-γ, GM-CSF, MIP-1α, MIP-1β, IP-10, MIG, Eotaxin, RANTES, and MCP-1 in serum, plasma, tissue culture supernant or cell lysates. The assay may be run alone or in combination with other Singleplex Bead Kits from Invitrogen. Check out our cytokine twenty-five-plex assay page on our website for more information. We also offer Human, Rat, and Mouse cytokine assays in ten-plex format.
Table 1: Analytes and their sensitivities (pg/ml) included in the twenty-five-plex cytokin assay kit. *Available as ten-plex assay |
    Figure 3: Typical standard curves for a Human cytokine twenty-five-plex (Note: Data collected as Twenty-five-plex but depicted in four multiplex groupings for clarity.) Data Courtesy of Invitrogen. |
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Fluorokine MMP Panel [Pricing] | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|
Developed based on the patented Luminex xMAP technology, by R&D sytems, the Fluorokine MMP assay provides simultaneous quantitation of total (Pro, Active, and TIMP bound forms) MMPs 1, 2, 3, 7, 8, 9, 12, and 13 in a single small sample volume. The multiplex Fluorokine MMP assays are extremely reliable with high sensitivity (See Table 2), wide range of detection, and very little cross reactivity. We offer quantitation services for the MMPs in serum, plasma, cell lysates, or tissue culture samples at extremely affordable prices, using R&D systems' calibrated MMP kit. For more information on the MMP multiplex assay, please check MMP assay page on R&D systems website.
|
Table 2: Analytes and their sensitivities (pg/ml) included in the Fluorokine MMP panel assay. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Additional Multiplex Assays | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|
Akt Pathway Total 7-Plex Panel
Analytes: IR,IGF-1R, IRS-1, Akt, PRAS40, p70S6K, and GSK-3β |
Death Receptor Human 3-Plex Panel
Analytes: TNF-RI, TNF-RII, and DR5 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|
Chemokine Human 10-Plex Panel
Analytes: Eotaxin, GRO-α, IP-10, MIG, MCP-1, MCP-2,MCP-3, MIP-1α, MIP-1β, and RANTES |
Cytokine Mouse 10-Plex Panel
Analytes: IL-1β, IL-2, IL-4, IL-5, IL-6, IL-10, IL-12, IFN-γ, TNF-α, and GM-CSF | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|
Growth Factor Human 4-Plex Panel
Analytes: VEGF, EGF, FGF basic, and G-CSF |
Neuroscience Cytokine 4-Plex Panel
Analytes: IL-1β, IL-6,IL-8, and TNF-α in cerebrospinal fluid (CSF) |
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Ocean Ridge Biosciences 10475 Riverside Drive, Suite 1 Palm Beach Gardens, FL 33410 |
Updated 03-09-10 |
Phone: 561-427-7845 |
|
microarray microarray service microarray services oceanridgebio ocean ridge ORB microarray ORB ocean ridge biosciences microRNA microRNA microarray microRNA array microRNA service miRNA miRNA microarray miRNA array miRNA service micro-RNA mi-RNA
|