ORB provides accurate detection and quantification of known and novel microRNAs, piRNAs and several other non-coding RNAs, such as scaRNA, snRNA, snoRNA, and vault RNA; profiling up to 60 million small RNAs per sample by capitalizing on Illumina's new Truseq chemistry and high throughput sequencing technology. ORB can also multiplex small RNA samples on the instrument for a low cost solution and also offers low input service for researchers with minimal amounts of starting material.
ORB uses Illumina's sequencing by synthesis (SBS) technology with TruSeq chemistry for generating unbiased cDNA libraries of small RNA from 1 - 10 ug of total RNA. ORB's standard service offers multiplexed coverage for microRNAs, piRNAs and other non-coding RNAs in the size range of 20-30 bases from 2 ug of total RNA. ORB also offers a proprietary low input protocol for small RNA sequencing from just 30 nanograms of total RNA for researchers working on rare samples such as laser captured cells, flow sorted cells, micro-needle aspirates, micro-dissected tissue and embryonic tissue. Our unparalleled sequencing service package is very competitively priced too! Please use the ORB Contact Form to obtain more information or request a quote for your project.
Highlights
- Wide coverage of various small RNA categories
- Illumina's Truseq chemistry or proprietary low input technology
- Multiplexing for low cost solution
- Competitively priced complete service package
- Complete bioinformatics support including statistical analysis
Service package includes:
- RNA sample quality control and report
- Library preparation
- Data QC and Report
- Complete bioinformatics analysis and report, which includes assembly & alignment to reference, FASTQ files of trimmed sequences, small RNA counting, statistical analysis, and clustering.
Small RNA Sequencing from Biofluids
ORB has the capability of performing accurate detection, quantification and sequencing of microRNAs from biofluid samples like plasma and serum. This capacity allows ORB to support a wide range of research projects dedicated towards biomarker discovery and RNA profiling.
ORB’s biofluid small RNA sequencing service has direct applications in biomarker discovery as microRNAs are considered to be an ideal class of blood-based biomarkers for cancer detection. Even though the total RNA content in plasma and serum is very low (1-10 ng/ml), the exceptional stability of microRNA in biofluids makes it an important class of biomarker for detection and prognosis of various types of cancers like prostate, lung and colon cancer.
ORB’s ability to isolate circulating microRNAs from biofluid samples like saliva, CSF, lymphatic fluid, and lavage provides researchers from a wide range of scientific backgrounds like cardiology, neuroscience, oncology, reproductive sciences, and molecular diagnostics with the capacity to engage in focused biomarker discovery and development projects.
See more on Biofluid Profiling
RNA requirement for small RNA sequencing projects
Illumina TruSeq small RNA protocol (Standard)2 microgram
Proprietary low input protocol30 nanogram
ORB can customize the size range for your small RNA discovery project for any small RNA ranging from 20 - 250 bases. Refer to the Table 2 to select the small RNA categories of your interest.
Average length for various small RNA types
7SK RNA271
anti-CLST11240 RNA144
BC200 RNA174
FMR1P binding RNA269
miRNA20
scAlu RNA120
scaRNA200
snoRNA107
snRNA131
SRP 7SL RNA260
vault RNA90
Y RNA95
piRNA30