Biofluid Profiling Illumina Sequencers Biomarker Discovery Companion Diagnostics Predictive Modeling

Examining Genetic Variants with Liquid Biopsy and Tumor Samples

Ocean Ridge Biosciences provide ultra-deep sequencing of cancer genes (e.g. BRCA1, TP53 and PTEN) as well as cancer-related genes included in oncopanels of leading industry manufacturers e.g. Illumina and Swift Biociences (Table 1). The targeted genes in these oncopanels are among the most studied genes in cancer research. This cost-effective ultra-deep sequencing approach allows direct analysis of potentially pathogenic genetic variants residing in known oncogenes. In addition, ORB can isolate cell-free DNA (cfDNA) from patient blood samples, and genomic DNA from tissue biopsies for ultra-deep sequencing of targeted oncogenes. The focused nature of oncopanels can reduce costs and increase the speed of genomic studies because only a limited set of genes are interrogated. Additional benefits include advanced detection of low-frequency variants with deeper sequencing options and production of more manageable datasets than traditional whole genome sequencing studies.


ORB’s Oncogene Analysis Service

A wide choice of oncopanels are available for selection at ORB for oncogene analysis service (Table 1). Many of the oncopanels accommodate low input DNA (10 ng) and are therefore amenable to cfDNA oncogene analysis. While some oncopanels include sets of genes involved in various tumor types, others are more specific and contain genes involved in specific cancer types e.g. colorectal cancer and lung cancer. The diversity as well as the specificity of the oncopanels available for ORB oncogene analysis service enable selection of the best oncopanel that satisfies your research interests.

Click here to view a sample report detailing germline mutations associated with increase risk of breast cancer utilizing Accel-Amplicon BRCA1 and BRCA2 oncopanel

Common Oncology Areas Utilizing Oncopanels
  • Breast cancer
  • Melanoma
  • Colorectal cancer
  • Pancreatic cancer
  • Ovarian cancer
  • Prostate cancer
  • Non-small cell lung cancer
  • Renal cell carcinoma
Table I. Oncopanels available for analysis using ORB's oncogene analysis service.
Panel Genes Cancer Type Manufacturer FFPE cfDNA oncogene analysis
  TruSight Cancer Sequencing 94   Pan-Cancer Illumina    
  TruSight Tumor 15 15   Solid Tumor Illumina Yes  
  TruSight Myeloid Sequencing Up tp 54   Hematological Illumina    
  AmpliSeq - Cancer Hotspot Panel v2 48   Pan-Cancer Illumina Yes  
  TruSight Tumor 170 170   Solid Tumor Illumina Yes  
  AmpliSeq - Custom DNA Panel Up to 12,000 amplicons   Pan-Cancer Illumina Yes  
  TruSight Oncology 500 523   Pan-Cancer Illumina Yes Yes
  Accel-Amplicon 56G Oncology Panel v2 56   Multiple Swift Biosciences Yes Yes
  Accel-Amplicon Plus 57G Pan-Cancer Profiling Panel 57   Pan-Cancer Swift Biosciences   Yes
  Accel-Amplicon BRCA1 and BRCA2 2   Breast and Ovarian Cancer Swift Biosciences Yes Yes
  Accel-Amplicon BRCA1, BRCA2, and PALB2 3   Breast and Ovarian Cancer Swift Biosciences Yes Yes
  Accel-Amplicon Comprehensive TP53 1   Multiple Swift Biosciences Yes Yes
  Accel-Amplicon EGFR Pathway 4   Multiple Swift Biosciences Yes Yes
  Accel-Amplicon™ Plus Colorectal (CRC) Cancer Panel 16   Colorectal Cancer Swift Biosciences Yes Yes
  Accel-Amplicon™ Plus Lung Cancer Panel 17   Lung Cancer Swift Biosciences Yes Yes
  Accel-Amplicon™ Plus Myeloid Panel 23   Hematological Swift Biosciences Yes Yes
  xGen®Acute Myeloid Leukemia Cancer Panel >260   Acute Myeloid Leukemia IDT    
  xGen®Pan-Cancer Panel 127   Pan-Cancer IDT    
  Human Breast Cancer Panel 93   Breast Cancer Qiagen Yes  
  Human Colorectal Cancer Panel 71   Colorectal Cancer Qiagen Yes  
  Human Lung Cancer Panel 72   Lung Cancer Qiagen Yes  
  Human Myeloid Neoplasms Panel 141   Hematological Qiagen Yes  
  Human Actionable Solid Tumor Panel 23   Solid Tumor Qiagen Yes  
  Human BRCA1 and BRCA2 Panel 2   Breast and Ovarian Cancer Qiagen Yes  
  Human BRCA1 and BRCA2 Plus Panel 6   Breast and Ovarian Cancer Qiagen Yes  
  Human Comprehensive Cancer Panel 160   Multiple Qiagen Yes  
  SeqCap EZ Comprehensive Cancer Design 578   Multiple Roche    
  SeqCap EZ Human Oncology 981   Multiple Roche    
  CleanPlex® OncoZoom Panel 65   Multiple Paragon Genomics Yes Yes

Contact Us to learn how ORB’s oncopanel service can facilitate a deep view of the genetics in your oncology research!

Sample Types and Sample Submission Instructions
  • Tumor biopsies
  • Genomic DNA (gDNA)
  • Cell-free DNA (cfDNA)
Sample Type Minimum Input Requirement Sample Submission Checklist
Tumor tissue 10 mg Tissue
Genomic or cell-free DNA 10 ng gDNA / cfDNA
Biofluid 4 ml Biofluid
ORB provides cfDNA extraction services with blood-based biofluid samples and genomic DNA isolation from tumor tissues. ORB is a Biosafety Level 2 (BSL-2) laboratory, and can accept samples that contain agents associated with human diseases.

For more information on sample submission please click here! For further questions please email or call 754-600-5128 .

Contact us to determine which panel would be most appropriate for your cancer investigation!